Pengembangan Biakan in-Vitro Plasmodium falciparum secara Kontinu

tuti, Sekar (1994) Pengembangan Biakan in-Vitro Plasmodium falciparum secara Kontinu.

Abstract

To support malaria research on its serology/immunology, chemotherapy, drug sensitivity aspects etc. especialy for falciparum malaria, a large amonut of antigen (parasites) is needed. These antigen could not be obtained from patients in the field only. Considering this situation, attempts have been made to develop a Plasmodium falciparum continuous culture in-vitro following a method introduced by Trager and Jensen (1976). In our laboratory, the parasite grew and multiplied nicely for 60 days. During that period of cultivation, a large amount of parasites (mostly mature trophozoite and schizont stages) have been collected for antigen production. Several tubes of mostly young trophozoite stage have been preserved, it can be culture again in th future or transported to another laboratory for futher culture.

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