Status Gizi Dan Fungsi Hati Mencit (Galur CBS-Swiss) Dan Tikus Putih (Galur Wistar) Di Laboratorium Hewan Percobaan Puslitbang Biomedis Dan Farmasi

Sihombing, Marice (2010) Status Gizi Dan Fungsi Hati Mencit (Galur CBS-Swiss) Dan Tikus Putih (Galur Wistar) Di Laboratorium Hewan Percobaan Puslitbang Biomedis Dan Farmasi. Media Penelitian dan Pengembangan Kesehatan, 20 (1). ISSN 0853-9987

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Abstract

Mice (CBS-Swiss strain) and albino rats (Wistar strain) are experimental animals bred and kept in Centre for Biomedical and Pharmaceuticals Research and Development, National Institute of Health Research and Development (NIHRD) Jakarta. The objective of this study is to elucidate the health profile of these animals and to assess their suitability as experimental animals. Parameters being measured include body weight gain, histopathology of the liver, blood test (SGPT, SGOT, billirubin scale) and internal organ weights (liver, kidney, lungs and lymph nodes). In this study, particularly for the body weight, used 180 mice and 180 rats, divided into 3 age groups 1, 2 and 3 months old. Each age groups consisted of 60 animals (30 male and 30 female). For the measurement of blood test, weight of internal organs and histopathologic of the liver examination used 60 mice and 60 rats from these animals. Which are divided into 3 groups 1, 2 and 3 months old, each groups were composed of 20 (10 male and 10 female animals). Mice and rats are taken at random sampling. Data analysis were conducted by ANOVA one-way test, except for organ weight (liver and lungs for mice and liver for albino rats) where data were analysed using a non-parametric test. Linear regression test was conducted for measurement of body weight gain in each age group. The result of the study suggested that the weight gain in mice and albino rats correlate with increasing of age. Histopathological assessment of liver did not show any cell damage or degeneration of parenchyma cells in both species. The blood test also showed normal values, and in general the organ weights (liver, kidney, lungs and lymph nodes) showed an increase that correlated with age. The study conclude that in general the conditions of these animals and their physiological state are good, and they are fit to be used as experimental animals.

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