Uji Serologi Isolat Bacillus thuringiensis dan Patogenisitasnya terhadap Jentik Nyamuk Vektor

Ch. P., Blondine (1999) Uji Serologi Isolat Bacillus thuringiensis dan Patogenisitasnya terhadap Jentik Nyamuk Vektor. Buletin Penelitian Kesehatan, 27 (2). ISSN 0125-9695

Abstract

Serology test study of 20 Bacillus thuringiensis bacteria isolates and it's pathogenicity against vector mosquito larvae last instar III; on samples from various soil habitat was done in Salatiga municipal, Semarang regency, Purworejo regency and East Flores regency. The method used in this study (to determine Bacillus thuringiensis isolate serotype) was based on H-antigen (flagela) which is used as a movement organ of the bacteria. The twenty Bacillus thuringiensis isolates which were tested; can be grouped to II serotype (serotype H-3, 14, 43, 10, 8, 24, 11, 6, 23, 28, 13 and 11). H-14 serotype is the dominant serotype (3 isolate) followed by H-3, 43, 10 and 23 serotype are 2 isolate respectively and H-8, 24, II, 6; 28 and 13 serotype are 1 isolate respectively. The pathogenicity test of 3 Bacillus thuringiensis isolate H-14 serotype against Aedes aegypti and Culex quinquefasciatus mosquito larvae, showed that 3 isolates (100%) has pathogenecity at 82.7%-94.7% and 64.0%-93.3% for 24 hours of exposure respectively. In 48 hours of exposure, using the same test, results showed that 3 isolates (100%) have a pathogenicity at 84.0%-98.7% and 81.3%-96: 0% respectively.

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