Dengue infection has expanded to other geographic areas in Indonesia due to changes in human ecology and behavior. In many tropical and subtropical areas of the world this viruses are endemic and causes periodic or annual outbreaks of disease. Among the complex and multifactors responsible for the increase of dengue cases, the transovarian mechanism may play an important role. This research was conducted to investigate the posibility of transovarian transmission of dengue virus in the population of Aedes aegypti and Ae. albopictus in endemic areas of Central Java Province. Aedes aegypti and Ae. albopictus larvae were collected from the DHF endemic areas i.e., Semarang Municipality, Kendal, Tegal, Grobogan and Sukoharjo Regency. Aedes sp. larvae from endemic areas were reared to adults in Insectary of Vector and Reservoir Diseases Research and Development Unit Salatiga. Head squashes of either male or female of all Aedes sp. mosquito (Ae. aegypti and Ae. albopictus) were tested for presence of dengue antigen in the brain based on immunohistochemical (streptavidin-biotin-peroxidase-complex) techniques using monoclonal antibody against dengue antigen (1 C 7) produced by Gadjah Mada University. Results indicated that Ae. aegypti population (0, 48% - 8, 77%) from 6 distric of 5 endemic area (Ngemplak Simongan and Manyaran Distric, Semarang Municipality, Cepiring and Kaliwungu District Kendal Regency, Ngruki and Makamhaji Grogol District, Sukoharjo Regency) have brownish color in their brain, meanwhile others have blue color. It was concluded that the population ofAe. aegypti from 6 distric of 5 endemic area have shown the transovarian transmission of• dengue virus. Brownish color was also [bund on the Ae. albopictus fi-om Manyaran District Semarang Municipality, therefore it was proved to be a dengue vector. TherefÖre vector control activities should focus on larval stages using larvicide or regular household sanitation or clean-up campaigns. The 3 M slogan which in English translates to dry' off, cover and bury nzust be added accordingly ./br getting rid o/• mosquito 's eggs (brush 0//) M'hich are attached to the surface containers (3M + S); this mnust he i'!/örnzed soon to the conununitv.