Andrographolide is the main constituent of Andrographis paniculata Ness (AP) which was poorly soluble in water and bitter taste. As diterpenoid lactone andrographolide has many biological activities, some of them are immunostimulant, anti malaria, hepatoprotector and anti mutagenic. The objective of this study were isolation and identification andrographolide from AP. In this study AP metanol extracted by percolation. Filtrate was collected and congealed ad viscous and was shaked by ethylacetate - water (l:l). Ethylacetate fraction was congealed until crystal formed. Recrystallization with hot metanol repeatedly until pure andrographolide isolate. Identification of andrographolide isolate by thin layer chromatography (TLC), UV spectrophotometer, Nuclear Magnetic Resonance (NMR) and Fourier Transform Infra Red. Result of this study showed that from one kilogram AP herbs dried is produced 0,43% andrographolide isolate as identic as andrographolide standard (Sigma). The continued research to develop phytopharmaca product that consist andrographolide or AP herbs is needed to minimize taste bitter or to increase solubility is water so that its useful in health services.