Pengaruh Konsentrasi Antimikrooganisme Media Fermentasi dan Waktu Inkubasi Terhadap Pertumbuhan Absidia corymbifera (Cohn) Sacc. & Trotter Dari Jamur Endofit Fusarium nivale

Suciatmih, Suciatmih Pengaruh Konsentrasi Antimikrooganisme Media Fermentasi dan Waktu Inkubasi Terhadap Pertumbuhan Absidia corymbifera (Cohn) Sacc. & Trotter Dari Jamur Endofit Fusarium nivale. Media Penelitian dan Pengembangan Kesehatan, 20 (1). pp. 17-25. ISSN 0853-9987

Text 162652-pengaruh-konsentrasi-antimikroorganisme-64c96cb5.pdf Download (575kB)

Abstract

Fusarium nivale (Fr.) Ces., an endophytic fungi from Dendrobium cremenatum Sw. (pigeon orchird) showed an antimicroorganism activity against Absidia corymbifera (Cohn) Sacc. & Trotter, causing of human zygomycosis. The aim of this study is to find out the effect of concentration of antimicroorganism agent, fermentatio medium, and incubation time control A corymbifera by F. nivale; and to make curve of F. nivale growth and curve of its antimicrobial production. Five different concentrations of antimicroorganism agent (0, 75, 150, 225 and 300 ul) four different fermentation media (Potato Dextrose Yeast, Tauge Exctract Broth, Kedelai Extract Broth, and Jagung Extract Broth) and eight different incubation times (0, 1, 2, 3, 4, 5, 6.and 7 days) were chosen for this experiment. Parameters of the study were change of filtrate pH, dry weight of mycelial cell of F. nivale and percentage inhibition of mycelial growth of A. cotymbifera. The results indicated that antimicroorganism agent concentration of 150 ul aplied and potato dextrose yeast medium were the most effective to inhibit A. corymbifera. The antimicroorganism agent was produced during growth of the fungi. The production of antimicroorganism agent coined with the decrease in pH from 6.0 to 4.81; and the increase in pH from 4.81 to 7.24-8.01. a optimum activity of antimicroorganism agent (31,23) during growth of fungi occurred on the 2th day of incubation

Actions (login required)

View Item