IDENTIFIKASI DAN PENETAPAN KADAR SENYAWA KUMARIN DALAM EKSTRAK METANOL Artemisia Annua L. SECARA KROMATOGRAFI LAPIS TIPIS - DENSITOMETRI

Alegantina, Sukmayati and Isnawati, Ani (2010) IDENTIFIKASI DAN PENETAPAN KADAR SENYAWA KUMARIN DALAM EKSTRAK METANOL Artemisia Annua L. SECARA KROMATOGRAFI LAPIS TIPIS - DENSITOMETRI. Buletin Penelitian Kesehatan, 38 (1). ISSN 0125-9695

Preview

Text 119-321-1-PB.pdf - Published Version Download (693kB) | Preview

Abstract

Kumarin and its derivatives have biological activity that can stimulate skin pigment, blood anticoagulation and can inhibit the effects of carcinogens. With this biological activity of kumarin, the research is done to ensure there is kumarin by identification and measure kumarin level which is contained in the Artemisia annua L.herb. The analysis methods include the extraction and fractionation. Identification and determination of level with Thin-Layer Chromatography (TLC) using a Densitometer CS-9301 PC. From the result of TLC identification of kumarin standard known that Artemisia annua L extract contain kumarin compound which marked by a blue spot flouresense on standards and methanol extract of artemisia annua L. seeing under UV light at a wavelength of 366 nm with Rf value of standard and sample is 0.31, the measurement of kumarin spot with Densitometer known that kumarin concentration in the extract of Artemisia annua L. is 10.5 ul / ml with 105% Recovery

Actions (login required)

View Item