Pengendalian Jentik Nyamuk Vektor Demam Berdarah, Malaria dan Filariasis Menggunakan Strain Lokal Bacillus thuringiensis H-14

Ch. P., Blondine (1999) Pengendalian Jentik Nyamuk Vektor Demam Berdarah, Malaria dan Filariasis Menggunakan Strain Lokal Bacillus thuringiensis H-14. Buletin Penelitian Kesehatan, 27 (1). ISSN 0125-9695

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Abstract

The Mosquito Larvae Control of Dengue Haemmoraghic Fever, Malaria and Filariasis Vector Using Local Strain of Bacillus thuringiensis H-14 A study was conducted anticipating vector control for the control of Dengue Haemmoraghic Fever (Aedes aegypti), malaria (Anopheles aconitus) and filariasis (Culex quinguefasciatus) using a local strain of Bacillus thuringiensis H-14 grown in local media (coconut water and soybean infusion). Tryptose Phosphate Broth chemical media (standard media) was used as a comparison to the media under investigation. Good growth was obtained in all media (local media and standard media) and local strain of B. thuringiensis H-14 was effective against the three mosquito larvae. The local strain of Bacillus thuringiensis H-14 cultured in coconut water media, killed 50% of the third instar larvae of Ae. aegypti, An. aconitus and Cx. quinquefasciatus at concentrations of 13 x 10-5, 31 x 10-5 and lOx 10-5 concentrations for 24 hours of exposure respectively and 13 x 10-5, 9 x 10-5 and 7 x 10-5 at 48 hours exposure. Meanwhile when B. thurigiensis H-14 were cultured in soybean infusion media, they killed 50% of the third instar larvae of Ae. aegypti, An. aconitus and Cx. quinquefasciatus at concentrations of 5 x 10-5, 7 x 10-5 and 4 x 10-5 for 24 hours of exposure respectively and 3 x 10-5, 5 x 10-5 and 3 x 10-5 at 48 hours exposure. However when they were cultured in TPB media, they killed 50% of the third instar larvae of Ae. aegypti, An. aconitus and Cx. quinquefasciatus at concentrations of 12 x 10-5, 15 x 10-5 and 3 x 10-5 for 24 hours of exposure respectively and 12 x 10-5, 15 x 10-5 and 2 x 10-5 at 48 hours exposure. This investigation shows that the local strain of B. thuringiensis H-14 has potential as bioinsecticide agent.

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